Services

The BioSkryb Advantage

Single cells.  Amplified accuracy.  Unlimited potential.

Created by a scientist who felt his own cancer research was being limited by existing genome amplification methods, BioSkryb’s proprietary PTA technology provides the required breadth and specificity to define cellular heterogeneity at the level of the most basic building blocks of life - the single cell.  Our mission is to support the true visionaries, those who entered the field of science because they want to make transformative discoveries that improve human health.

We offer premium products and services at a competitive price and are here to assist you at any stage in your workflow, from amplification of single cells through data analysis or anywhere in between.  We will work with you to customize a true service solution that optimizes and transforms your scientific discoveries.  We are here to help you streamline your process, to let every cell tell its story.

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Whole Genome Amplification

Whether you don't have enough time or resources in your busy lab or just want to ensure success with our novel technology, leverage BioSkryb's experience with Primary Template-directed Amplification by sending us your FACS sorted cells for WGA. Our expert team will run ResolveDNA™ Whole Genome Amplification on your samples with our custom PTA consumables and equipment to maximize results.

Library Preparation

Once the samples go through the PTA process, sizing and yield of the PTA products will be ascertained by our TapeStation and Qubit Fluorometer. Our specially formulated ResolveDNA™ Library Preparation Kit will be used for preparing Illumina libraries using BioSkryb’s ResolveDNA™ Multi-Use Library Adapters. The fragment sizing and yield of Illumina libraries will be determined before sequencing to maximize results. Quality-controlled Illumina libraries can be submitted to BioSkryb’s incredibly competitively-priced sequencing pipeline, or we can ship quality-controlled libraries to you for your own sequencing solution.

 

 

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Sequencing

BioSkryb is excited to offer the lowest sequencing prices possible to enable you to increase your throughput. Our sequencing is performed using Illumina sequencers with 550 million paired-end reads at an industry-low $800 for an entire human genome. We offer an initial (optional) round of low-pass sequencing to identify cells that were dropouts or otherwise show signs of poor performance to ensure the most robust libraries move forward to deep sequencing. We guarantee access to Fastq files for 1-year from the date of sample acquisition.

BioSkryb TrailBlazer™ Bioinformatics Platform Beta Version

We offer a complimentary TrailBlazer™ Bioinformatics Platform Beta Version account to all customers that either purchase a kit or use BioSkryb's services. TrailBlazer performs all steps of a standard analysis and allows exploration of the resulting data. Users can use it to explore single-cell datasets, or to download aligned data for the application of custom workflows.

The TrailBlazer platform uses standardized best practices for bioinformatic DNA sequencing analysis to allow users to evaluate the quality of their data, including sequencing alignment, coverage, and single nucleotide variant calling metrics. The Beta Version of the analysis platform will also visualize the results in an intuitive layout, allowing users to compare results between cells (or samples).

The TrailBlazer Beta Version is compatible with Illumina, MiSeq, MiniSeq, NextSeq, and NovaSeq platforms. Future versions will incorporate the calling and visualization of additional types of genomic variants (including structural and copy number variants), as well as the integration of multi-omics data.